An educational illustration depicting the process of exocytosis
Impact of Crocetin on Autophagy: Infographic on Cell Induction Effects
@proficient-coral31 creation ·
Explore our detailed infographic showcasing the concentration- and time-dependent effects of Crocetin on autophagy induction in N9 microglial cells and primary neurons. Discover key data, visualized graphs, and markers illustrating the increase of LC3B-II and the decrease of SQSTM1/p62 levels post-treatment.
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In Vitro Evidence: Crocetin Induces Autophagy in Cells** #### **Concentration- and Time-Dependent Autophagy Induction** - **Data**: Crocetin treatment increases the levels of **LC3B-II** (a marker of autophagosome formation) and decreases **SQSTM1/p62** (a protein degraded during autophagy) in a **concentration-dependent** manner in N9 microglial cells and primary neurons. - **LC3B-II levels**: Increase from 3.12 µM to 50 µM crocetin. - **SQSTM1/p62 levels**: Decrease with increasing crocetin concentrations. - **Time-Dependence**: Autophagy markers (e.g., LC3B-II, ATG5, ATG7) increase over time (3 h to 24 h) after crocetin treatment. - **Relevance**: This shows that crocetin induces autophagy in a dose- and time-dependent manner, confirming its pharmacological activity.
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