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1. During the ritual, the priest performed ligation to ensure that the blood vessel remained closed and prevented any bleeding.
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2. The painting depicted a surgeon delicately performing a ligation to secure the blood vessel and maintain the integrity of the artwork.
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3. In a gesture of love, the surgeon performed the ligation on his partner's injured duct, symbolizing their commitment to each other.
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4. In the animated series, the character used ligation to comically exaggerate the process of stopping bleeding, resulting in comedic effects.
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